Imaging Cycler® basic

a cost-effective 4-in-one microscope

It combines:

Optional: many visual fields.

The Imaging Cycler® TIS™ -HTS-Live

a 6-in-one microscope

It combines:

For note: Both Imaging Cycler®-basic and Imaging Cycler® TIS™ -HTS-Live are fully automated microscopes that can be also used in conventional non-automated modes for routine daily microscopic analyses as well as for pre-programmed protein network (toponome) imaging. Imaging Cycler® comes with remote control.

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Functional super-resolution

Standbild Zelle

 

Imaging Cycler® 3D functional super-resolution of interlocked multi-protein clusters at the cell surface of a human blood T lymhocyte. Note: Colours denote the differential assembly of 27 distinct co-mapped membrane proteins ( = protein colocation and anti-colocation code). (after: Friedenberger M et al: Nat Protoc 2007, 2, 2285-84, Schubert W. et al. N Biotechnol 2012)

Simultaneous functional super-resolution of protein networks and super-resolution of structures

ttifm

Conventional triple fluorescence mode of Imaging Cycler® TIS™. Dermoepithelial junction, 200 nm resolution

ttifm2

Imaging Cycler® TIS™ -100 simultaneous paramaters. Dermoepithelial junction, same visual field as above, 40nm resolution

TEM

Transmission electron micrograph, showing: BC: basal epithelial cells; LL: lamina lucida of the basal lamina, BL (width appr 40 nm); LD: lamina densa of the BL; LF: lamina fibroreticularis of the BL. NOTE: these three layers of the basal lamina are clearly distinguished by 100-parameter Imaging Cycler® TIS™ (above) substantiating that simultaneous 100-paramater Imaging Cycler® TIS™ imaging simultaneously distinguishes both the basal lamina substructures and the protein networks which are unique for these different BL layers. (after: Schubert W et al. N Biotechnol 2012).

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